Genetic diversity of dry bean (Phaseolus vulgaris) accessions of Kenya using AFLP markers
AFLP technique was applied to evaluate the genetic diversity among 35 common beans varieties of market class drawn from four populations. Two AFLP primers produced 324 polymorphic loci with an estimated variance of 53.45 and a within population variance of 83%. The pair wise matching indices (SM) based on the AFLP profiles were subjected to UPGMA cluster analysis. The pair wise matching coefficients among P. vulgaris generated a dendogram with the similarity coefficient values ranging from 0.66 to 0.89. Neisâ€™ genetic Distance (Ds) among the four populations from which the bean samples were drawn varied from 0.01 to 0.09. The genetic distance observed between the dry bean from the highlands and the dry lands was lower (0.0186), compared to those from western province and market centres (0.0326). From the analysis, moderate to high genetic diversity was observed. These findings can be used to guide future bean breeding studies and germplasm management in Kenya.
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